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Purpose This study aims to improve the passenger accessibility of passenger demands in the end-of-operation period. Design/methodology/approach A mixed integer nonlinear programming model for last train timetable optimization of the metro was proposed considering the constraints such as the maximum headway, the minimum headway and the latest end-of-operation time. The objective of the model is to maximize the number of reachable passengers in the end-of-operation period. A solution method based on a preset train service is proposed, which significantly reduces the variables of deciding train services in the original model and reformulates it into a mixed integer linear programming model. Findings The results of the case study of Wuhan Metro show that the solution method can obtain high-quality solutions in a shorter time; and the shorter the time interval of passenger flow data, the more obvious the advantage of solution speed; after optimization, the number of passengers reaching the destination among the passengers who need to take the last train during the end-of-operation period can be increased by 10%. Originality/value Existing research results only consider the passengers who take the last train. Compared with previous research, considering the overall passenger demand during the end-of-operation period can make more passengers arrive at their destination. Appropriately delaying the end-of-operation time can increase the proportion of passengers who can reach the destination in the metro network, but due to the decrease in passenger demand, postponing the end-of-operation time has a bottleneck in increasing the proportion of passengers who can reach the destination.